CRP、Procarcitonin、といったBiomarker嫌いの編集長も今回は少し心が動いた事を正直に告白致します。
HIV感染症の治癒を難しくしている根本は、CD4陽性Tリンパ球に「潜む」HIVが除去出来ない事にあります。どこに潜んでいるか、どのCD4に潜んでいるか分かれば・・治癒に至かも・・
そこで今回のBiomarker、「CD32a」です。これは積極的に増殖しているCD4に認められない、しかし、恐らく「Latent」である事の表現としてUpregulateされるマーカーなのですな・・(多分）
タイトル写真：ジドブジン（レトロビル）のみでHIV感染症と戦っていた頃の編集長
詳細は以下をどぞ
Nature. 2017 Mar 15. doi: 10.1038/nature21710.
CD32a is a marker of a CD4 T-cell HIV reservoir harbouring replication-competent proviruses.
Discours Bら
Abstract
The persistence of the HIV reservoir in infected individuals is a major obstacle to the development of a cure for HIV. Here, using an in vitro model of HIV-infected quiescent CD4 T cells, we reveal a gene expression signature of 103 upregulated genes that are specific for latently infected cells, including genes for 16 transmembrane proteins. In vitro screening for surface expression in HIV-infected quiescent CD4 T cells shows that the low-affinity receptor for the immunoglobulin G Fc fragment, CD32a, is the most highly induced, with no detectable expression in bystander cells. Notably, productive HIV-1 infection of T-cell-receptor-stimulated CD4 T cells is not associated with CD32a expression, suggesting that a quiescence-dependent mechanism is required for its induction. Using blood samples from HIV-1-positive participants receiving suppressive antiretroviral therapy, we identify a subpopulation of 0.012% of CD4 T cells that express CD32a and host up to three copies of HIV DNA per cell. This CD32a+ reservoir was highly enriched in inducible replication-competent proviruses and can be predominant in some participants. Our discovery that CD32a+ lymphocytes represent the elusive HIV-1 reservoir may lead to insights that will facilitate the specific targeting and elimination of this reservoir.
PMID: 28297712
HIV感染症の治癒を難しくしている根本は、CD4陽性Tリンパ球に「潜む」HIVが除去出来ない事にあります。どこに潜んでいるか、どのCD4に潜んでいるか分かれば・・治癒に至かも・・
そこで今回のBiomarker、「CD32a」です。これは積極的に増殖しているCD4に認められない、しかし、恐らく「Latent」である事の表現としてUpregulateされるマーカーなのですな・・(多分）
タイトル写真：ジドブジン（レトロビル）のみでHIV感染症と戦っていた頃の編集長
詳細は以下をどぞ
Nature. 2017 Mar 15. doi: 10.1038/nature21710.
CD32a is a marker of a CD4 T-cell HIV reservoir harbouring replication-competent proviruses.
Discours Bら
Abstract
The persistence of the HIV reservoir in infected individuals is a major obstacle to the development of a cure for HIV. Here, using an in vitro model of HIV-infected quiescent CD4 T cells, we reveal a gene expression signature of 103 upregulated genes that are specific for latently infected cells, including genes for 16 transmembrane proteins. In vitro screening for surface expression in HIV-infected quiescent CD4 T cells shows that the low-affinity receptor for the immunoglobulin G Fc fragment, CD32a, is the most highly induced, with no detectable expression in bystander cells. Notably, productive HIV-1 infection of T-cell-receptor-stimulated CD4 T cells is not associated with CD32a expression, suggesting that a quiescence-dependent mechanism is required for its induction. Using blood samples from HIV-1-positive participants receiving suppressive antiretroviral therapy, we identify a subpopulation of 0.012% of CD4 T cells that express CD32a and host up to three copies of HIV DNA per cell. This CD32a+ reservoir was highly enriched in inducible replication-competent proviruses and can be predominant in some participants. Our discovery that CD32a+ lymphocytes represent the elusive HIV-1 reservoir may lead to insights that will facilitate the specific targeting and elimination of this reservoir.
PMID: 28297712